atcc 204305 Search Results


a fumigatus atcc 204305  (ATCC)
98
ATCC a fumigatus atcc 204305
A Fumigatus Atcc 204305, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/a fumigatus atcc 204305/product/ATCC
Average 98 stars, based on 1 article reviews
a fumigatus atcc 204305 - by Bioz Stars, 2026-03
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atcc 204305  (ATCC)
96
ATCC atcc 204305
10-ZOL inhibits squalene synthesis, causing the depletion of ergosterol and a fungicidal effect. ( A ) Squalene rescue assays. C. albicans SC5314, C. neoformans H99, and A. fumigatus <t>ATCC</t> <t>204305</t> were treated with 10-ZOL at the MIC or with a no-drug control (1% DMSO). Exogenous squalene was added at the indicated concentrations. EC 50 is the concentration of squalene that rescued 50% of fungal growth relative to the no-drug control. Data are the mean of three replicates ± SD. ( B ) Cell ergosterol content following treatment with FLC or with 10-ZOL at 0.25, 1, and 4× MIC, with and without exogenous squalene (128 µg/mL). Ergosterol concentration was calculated as a percentage of the total wet weight of treated fungi. Data are the mean of three technical replicates of each of three independent biological replicates ± SD. Treatments with or without squalene were compared by two-way ANOVA, and significant (<0.05) P -values are presented. ( C ) Time-kill assays for C. albicans SC5314 and C. neoformans H99 treated with a no-drug control (1% DMSO) or 10-ZOL at 0.25, 0.5, 1, 2, or 4× MIC. Data are the mean of four technical replicates of each of three independent biological replicates ± SD.
Atcc 204305, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/atcc 204305/product/ATCC
Average 96 stars, based on 1 article reviews
atcc 204305 - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier

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10-ZOL inhibits squalene synthesis, causing the depletion of ergosterol and a fungicidal effect. ( A ) Squalene rescue assays. C. albicans SC5314, C. neoformans H99, and A. fumigatus ATCC 204305 were treated with 10-ZOL at the MIC or with a no-drug control (1% DMSO). Exogenous squalene was added at the indicated concentrations. EC 50 is the concentration of squalene that rescued 50% of fungal growth relative to the no-drug control. Data are the mean of three replicates ± SD. ( B ) Cell ergosterol content following treatment with FLC or with 10-ZOL at 0.25, 1, and 4× MIC, with and without exogenous squalene (128 µg/mL). Ergosterol concentration was calculated as a percentage of the total wet weight of treated fungi. Data are the mean of three technical replicates of each of three independent biological replicates ± SD. Treatments with or without squalene were compared by two-way ANOVA, and significant (<0.05) P -values are presented. ( C ) Time-kill assays for C. albicans SC5314 and C. neoformans H99 treated with a no-drug control (1% DMSO) or 10-ZOL at 0.25, 0.5, 1, 2, or 4× MIC. Data are the mean of four technical replicates of each of three independent biological replicates ± SD.

Journal: mBio

Article Title: Alkylation-based optimization of antifungal FPPS inhibitors yields a potent, broad-spectrum lipophilic zoledronate derivative

doi: 10.1128/mbio.03199-25

Figure Lengend Snippet: 10-ZOL inhibits squalene synthesis, causing the depletion of ergosterol and a fungicidal effect. ( A ) Squalene rescue assays. C. albicans SC5314, C. neoformans H99, and A. fumigatus ATCC 204305 were treated with 10-ZOL at the MIC or with a no-drug control (1% DMSO). Exogenous squalene was added at the indicated concentrations. EC 50 is the concentration of squalene that rescued 50% of fungal growth relative to the no-drug control. Data are the mean of three replicates ± SD. ( B ) Cell ergosterol content following treatment with FLC or with 10-ZOL at 0.25, 1, and 4× MIC, with and without exogenous squalene (128 µg/mL). Ergosterol concentration was calculated as a percentage of the total wet weight of treated fungi. Data are the mean of three technical replicates of each of three independent biological replicates ± SD. Treatments with or without squalene were compared by two-way ANOVA, and significant (<0.05) P -values are presented. ( C ) Time-kill assays for C. albicans SC5314 and C. neoformans H99 treated with a no-drug control (1% DMSO) or 10-ZOL at 0.25, 0.5, 1, 2, or 4× MIC. Data are the mean of four technical replicates of each of three independent biological replicates ± SD.

Article Snippet: Aspergillus fumigatus , ATCC 204305 , >2,048 , 256 , 256 , 128 , 512 , 64 , 0.63 , 128 , 32 , 0.75 , 64 , 16 , 0.38.

Techniques: Control, Concentration Assay

10-ZOL treatment causes hyperfluidity, depolarization, and leakage in fungal membranes. ( A ) Membrane hyperfluidity is expressed as the fluorescence anisotropy of TMA-DPH. C. albicans SC5314, C. neoformans H99, and A. fumigatus ATCC 204305 were treated with a no-drug control (1% DMSO) or 10-ZOL at 0.25, 0.5, 1, 2, or × MIC. ( B ) Fungal membrane depolarization is expressed as the changing emission λ max of diS-C 3 (3) over time. ( C ) Membrane permeabilization is measured by the amount of nucleic acid leaked into the culture supernatant. Data are the mean of three technical replicates of each of three biological replicates ± SD. Treatments were compared to the no-drug control (1% DMSO) by one-way ANOVA, and significant (<0.05) P -values are presented.

Journal: mBio

Article Title: Alkylation-based optimization of antifungal FPPS inhibitors yields a potent, broad-spectrum lipophilic zoledronate derivative

doi: 10.1128/mbio.03199-25

Figure Lengend Snippet: 10-ZOL treatment causes hyperfluidity, depolarization, and leakage in fungal membranes. ( A ) Membrane hyperfluidity is expressed as the fluorescence anisotropy of TMA-DPH. C. albicans SC5314, C. neoformans H99, and A. fumigatus ATCC 204305 were treated with a no-drug control (1% DMSO) or 10-ZOL at 0.25, 0.5, 1, 2, or × MIC. ( B ) Fungal membrane depolarization is expressed as the changing emission λ max of diS-C 3 (3) over time. ( C ) Membrane permeabilization is measured by the amount of nucleic acid leaked into the culture supernatant. Data are the mean of three technical replicates of each of three biological replicates ± SD. Treatments were compared to the no-drug control (1% DMSO) by one-way ANOVA, and significant (<0.05) P -values are presented.

Article Snippet: Aspergillus fumigatus , ATCC 204305 , >2,048 , 256 , 256 , 128 , 512 , 64 , 0.63 , 128 , 32 , 0.75 , 64 , 16 , 0.38.

Techniques: Membrane, Fluorescence, Control